Researchers at University of Tsukuba have investigated the structure and light energy transfer efficiency of a protein complex crucial to the photosynthesis of purple sulfur bacteria thriving in high-salt, high-alkaline environments.
Researchers at University of Tsukuba have investigated the structure and light energy transfer efficiency of a protein complex crucial to the photosynthesis of purple sulfur bacteria thriving in high-salt, high-alkaline environments. Cryo-electron microscopy observation and computer analysis revealed that this unique protein complex significantly enhances energy conversion ability.
Unlike plants and cyanobacteria, photosynthetic bacteria, such as purple sulfur bacteria, thrive in extreme environments with high salt concentrations and alkalinity. These bacteria use hydrogen sulfide (H2S) to convert solar into chemical energy. Light-harvesting protein complexes—specifically the light-harvesting two complex (LH2) and the core light-harvesting reaction center complex (LH1-RC)—play a crucial role in this process. Halorhodospira halophila, a purple sulfur bacterium, is believed to perform photosynthesis efficiently by integrating LH2 and LH1-RC. However, in nonsulfur bacteria, the interaction between LH2 and LH1-RC has been reported to be weak, and this key difference remains unclear.
To investigate this, researchers employed cryo-electron microscopy to observe LH2 and LH1-RC from Hlr. halophila at the amino acid level. Results revealed that LH1-LH2 and LH1-RC complexes are formed, the smallest unit of the LH1 structure is composed of an unusual polypeptide chain, and this LH1 structure surrounds LH2 or RC. Furthermore, experiments measuring intermolecular energy transfer showed that the LH1-LH2 complex achieves almost 100% light energy transfer efficiency, suggesting that its structural arrangement enhances energy conversion.
Read more at University of Tsukuba
Image: This illustration shows how two isoforms of the α and β chains in the LH1 subunit pair together to form a ring. The LH1 ring is structured to surround both the reaction center and the LH2 complex, which are depicted in semi-transparent colors on the left and right, respectively. (Credit: University of Tsukuba)
