Inserting or tweaking genes in plants is more art than science, but a new technique developed by University of California, Berkeley, scientists could make genetically engineering any type of plant—in particular, gene editing with CRISPR-Cas9—simple and quick.
Inserting or tweaking genes in plants is more art than science, but a new technique developed by University of California, Berkeley, scientists could make genetically engineering any type of plant—in particular, gene editing with CRISPR-Cas9—simple and quick.
To deliver a gene, the researchers graft it onto a carbon nanotube, which is tiny enough to slip easily through a plant’s tough cell wall. To date, most genetic engineering of plants is done by firing genes into the tissue—a process known as biolistics—or delivering genes via bacteria. Both are successful only a small percentage of the time, which is a major limitation for scientists seeking to create disease- or drought-resistant crops or to engineer plants so they’re more easily converted to biofuels.
Nanotubes, however, are highly successful at delivering a gene into the nucleus and also into the chloroplast, a structure in the cell that is even harder to target using current methods. Chloroplasts, which have their own separate, though small, genome, absorb light and store its energy for future use, releasing oxygen in the process. An easy gene-delivery technique would be a boon for scientists now trying to improve the efficiency of light energy capture to boost crop yields.
Read more at University of California – Berkeley
Graphic: A gene (red and white) bound to a carbon nanotube can easily diffuse into plant cells, where it is expressed as if it were the cell’s own gene. In this case, inserting the gene for green fluorescent protein makes the leaves glow green. The nanotube is 1 nanometer in diameter and several hundred nanometers long. Credit: Ella Marushchenko
